Introduction
Have you ever heard of 80 ammonium sulfate precipitation? If you haven’t, don’t worry! This article will explain everything you need to know about this process.
What is 80 Ammonium Sulfate Precipitation?
80 ammonium sulfate precipitation is a technique used to purify proteins. It involves adding ammonium sulfate to a protein solution until the solution is saturated. The protein then precipitates out of solution and can be collected through centrifugation.
Why is it Useful?
This technique is useful because it allows for the purification of proteins from complex mixtures. It can also be used to concentrate proteins and remove contaminants.
How Does it Work?
Ammonium sulfate is a salt that reduces the solubility of proteins. When added to a protein solution, the ammonium sulfate molecules interact with the water molecules and the proteins, causing the proteins to become less soluble and eventually precipitate out of solution.
The Process of 80 Ammonium Sulfate Precipitation
Step 1: Preparation
Before starting the process, make sure to have all necessary materials and equipment. This includes a protein solution, ammonium sulfate, a centrifuge, and appropriate containers for the precipitated protein.
Step 2: Adding Ammonium Sulfate
Slowly add ammonium sulfate to the protein solution while stirring gently. Keep adding until the solution is saturated with the salt. The saturation point can be determined by measuring the concentration of ammonium sulfate in the solution.
Step 3: Centrifugation
Centrifuge the solution at a high speed to separate the precipitated protein from the remaining solution. The protein will form a pellet at the bottom of the container.
Step 4: Collection
Carefully remove the supernatant (the liquid on top of the protein pellet) and collect the protein pellet. The pellet can then be resuspended in a buffer solution for further use.
Conclusion
80 ammonium sulfate precipitation is a useful technique for purifying proteins. It is a simple and cost-effective method that can be used in a variety of applications. By following the steps outlined in this article, you can easily perform 80 ammonium sulfate precipitation in your own lab.